Sub-diffraction resolution pump-probe microscopy with shot-noise limited sensitivity using laser diodes.
نویسندگان
چکیده
We demonstrate the use of intensity-modulated laser diodes to implement pump-probe microscopy and achieved sub-diffraction resolution imaging with shot-noise limited sensitivity with a scheme of balanced detection. This technique has several applications for various types of induced transmission change, including excited-state absorption, ground state absorption bleaching and stimulated emission. By using our technique, biological imaging of mouse T cells and the axons of neurons in the cerebral cortex was demonstrated.
منابع مشابه
Towards shot-noise limited diffraction experiments with table-top femtosecond hard x-ray sources
Table-top laser-driven hard x-ray sources with kilohertz repetition rates are an attractive alternative to large-scale accelerator-based systems and have found widespread applications in x-ray studies of ultrafast structural dynamics. Hard x-ray pulses of 100 fs duration have been generated at the Cu K α wavelength with a photon flux of up to 109 photons per pulse into the full solid angle, per...
متن کاملDefining the Noise-limited-resolution for Stimulated Emission Depletion Microscopy
With recent developments in microscopy, such as stimulated emission deletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we analyse how the interplay between shot noise, thermal noise, the saturation intensity of a fluorescent label, and the damage threshold of the sample changes the maximum resolution of...
متن کاملFar-field Imaging of Non-fluorescent Species with Sub-diffraction Resolution
Super-resolution optical microscopy is opening a new window to unveil the unseen details on the nanoscopic scale. Current far-field super-resolution techniques rely on fluorescence as the read-out1-5. Here, we demonstrate a scheme for breaking the diffraction limit in far-field imaging of non-fluorescent species by using spatially controlled saturation of electronic absorption. Our method is ba...
متن کاملFemtosecond photoelectron diffraction: a new approach to image molecular structure during photochemical reactions
Continuing technical advances in the creation of (sub-) femtosecond VUV and X-ray pulses with Free-Electron Lasers and laser-based high-harmonic-generation sources have created new opportunities for studying ultrafast dynamics during chemical reactions. Here, we present an approach to image the geometric structure of gas-phase molecules with fewfemtosecond temporal and sub-Ångström spatial reso...
متن کاملFemtosecond time-resolved optical pump-probe spectroscopy at kilohertz-scan-rates over nanosecond-time-delays without mechanical delay line
We demonstrate a technique for femtosecond time-resolved optical pump-probe spectroscopy that allows to scan over a nanosecond time delay at a kilohertz scan rate without mechanical delay line. Two mode-locked femtosecond lasers with approximately 1 GHz repetition rate are linked at a fixed difference frequency of fR=11 kHz. One laser delivers the pump pulses, the other provides the probe pulse...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Optics express
دوره 22 8 شماره
صفحات -
تاریخ انتشار 2014